The court challenge automatically cancels Mnangagwa's inaugartaion which had been scheduled to go ahead on Sunday the 12th of August.
The MDC alliance filed their paperwork with Zimbabwe's Constitutional Court on Friday, alleging the result of the July 30 vote had been rigged in favour of incumbent Mnangagwa, the leader of the ruling ZANU-PF party.
At the same time, Zimbabwe's Justice Minister Ziyambi Ziyambi said President Emmerson Mnangagwa's inauguration that had been set for Sunday had been deferred after opposition leader Nelson Chamisa challenged presidential election results in court.
Judges must rule on the case within 14 days and could declare a victor, call another election, or order a run-off or recount, the Veritas legal group said.
Mr Chamisa tweeted: "Our legal team successfully filed our court papers".
The MDC arrived at the court less than an hour before it closed on Friday, the last day that the petition could be lodged.
Hundreds of flights cancelled across Europe in Ryanair strike
French news source France Info dubbed the strike "historic", specifying that it is the biggest walkout in the history of Ryanair. Belgium-based Ryanair pilots gather at Charleroi Airport as part of a European-wide strike.
MDC Alliance lawyers say they want the election results overturned, they say the inauguration must not happen and they say some of the evidence they have are vital poll papers, V11 forms, that show an attempt to tamper with figures.
Also on Friday, lawyers for opposition figure Tendai Biti asked judges to throw out charges against him over the protests against alleged election fraud, in a case raising further global concern about the new government.
"The concerns on the welfare of Tendai Biti are foremost in the minds of many in the worldwide community; you have seen a statement from the EU, Canada, Australia and the US", he said while referring to an earlier statement by in which the western countries called on authorities to stick to the confines of the law in handling alleged political violations by the opposition.
Mnangagwa wrote on Twitter that Biti was released after he intervened personally in the case.
"The ZHRC has established that there is hunting down and harassment of polling agents for independent candidates and opposition political parties", it said.
Senior Zimbabwean opposition figure Tendai Biti was arrested as he tried to flee to neighbouring Zambia to seek asylum on Wednesday, his lawyer said, allegedly on charges of inciting violence over the disputed election result.